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Anti-VEGFA / VEGF Antibody (HRP) LS-C154110

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VEGFA / VEGF Antibodies

Anti-VEGFA / VEGF Antibody (clone VG76e) LS-C2929
Mouse Monoclonal [clone VG76e] (IgG1) to Human VEGFA / VEGF
Human, Bovine, Pig, Sheep
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Western blot Image
Anti-VEGFA / VEGF Antibody LS-C104581
Rabbit Polyclonal to Human VEGFA / VEGF
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-VEGFA / VEGF Antibody (clone VG-1) IHC-plus™ LS-B3579
Mouse Monoclonal [clone VG-1] (IgG1) to Human VEGFA / VEGF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human VEGFA / VEGF
Human
IHC, Western blot, ELISA
HRP Conjugated

Details

Human VEGFA / VEGF
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Horseradish Peroxidase
Ion exchange chromatography
Unmodified

Applications

  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA

Specificity and Use

VEGFA / VEGF antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kD in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
100 µl Reconstitute with 100ul deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About VEGFA / VEGF

P15692 NM_003376 NP_003367.4

VEGFA Antibody, VPF Antibody, Vascular permeability factor Antibody, VEGF Antibody, VEGF-A Antibody, MVCD1 Antibody

VEGFA / VEGF is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.

Western blot

Western blot
Peroxidase - Western Blot. Western Blot showing detection of Recombinant Human VEGF-165. 50 ng of protein (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20°C, Anti-VEGF-165 (RABBIT) Antibody Peroxidase Conjugate LS-C154110 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 19 kD molecular weight position expected for rH-VEGF-165. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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