Human VEGFA / VEGF
(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- Western blot (1:1000)
VEGFA / VEGF antibody was raised against this IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human VEGFA-121 protein produced in E. coli.
This antibody is specific for human VEGFA-121 protein. A BLAST analysis was used to suggest cross-reactivity with VEGFA-121 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to VEGFA-121 from a wide variety of animals. Cross-reactivity with VEGFA-121 from other sources has not been determined.
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14.7 Da in size corresponding to the mature human VEGFA-121 protein by western blotting in appropriate cell lysate or extract.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.