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Anti-VEGFA / VEGF Antibody LS-C104513

Note: This antibody replaces LS-C54320, LS-C42267, LS-C18445

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VEGFA / VEGF Antibodies

Anti-VEGFA / VEGF Antibody (clone VG76e) LS-C2929
Mouse Monoclonal [clone VG76e] (IgG1) to Human VEGFA / VEGF
Human, Bovine, Pig, Sheep
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Western blot Image
Anti-VEGFA / VEGF Antibody LS-C104581
Rabbit Polyclonal to Human VEGFA / VEGF
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-VEGFA / VEGF Antibody (clone VG-1) IHC-plus™ LS-B3579
Mouse Monoclonal [clone VG-1] (IgG1) to Human VEGFA / VEGF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse VEGFA / VEGF
Mouse
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Mouse VEGFA / VEGF
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (0.08 - 0.12 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

VEGFA / VEGF antibody was raised against e.coli derived recombinant Murine VEGF.
Mouse VEGF
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mVEGF (10.00 ng/ml), a concentration of 0.08-0.12 ug/ml of this antibody is required. ELISA: To detect mVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine VEGF (LS-C104524) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About VEGFA / VEGF

P15692 NM_003376 NP_003367.4

VEGFA Antibody, VPF Antibody, Vascular permeability factor Antibody, VEGF Antibody, VEGF-A Antibody, MVCD1 Antibody

VEGFA / VEGF is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.

Immunohistochemistry

Immunohistochemistry
IHC of VEGF antibody LS-C104513. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of VEGF antibody LS-C104513. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of VEGF antibody LS-C104513. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of VEGF antibody LS-C104513. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of VEGF antibody LS-C104513. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of VEGF antibody LS-C104513. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/6/2016

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