Human VEGFA / VEGF

Mouse

Human, Mouse, Rat, Dog, Rabbit (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

SPM225

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Human recombinant protein.

Human VEGFA / VEGF

• IHC
• IHC - Paraffin (2 - 4 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Optimal dilution of the VEGF antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15692 NCBI: NM_003376 NP_003367.4