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Catalog Number	Size	Price
LS-C355125-200	200 µg	$566

VEGFA / VEGF Antibody - Immunofluorescent analysis of Vascular Endothelial Growth Factor (VEGF, green) in Hela cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA for 30 minutes at room temperature. Cells were stained with (left panel) or without (right panel) a VEGF polyclonal antibody at a dilution of 1:40 for at least 1 hour at room temperature, and then incubated with a Dylight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:1000 for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.

VEGFA / VEGF Antibody - WB using VEGF-A Antibody (16F1)

VEGFA / VEGF Antibody - ELISA using VEGF-A Antibody (16F1)

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Monoclonal Mouse anti‑Human VEGFA / VEGF Antibody (clone 16F1, IF, WB) LS‑C355125

Name: Anti-VEGFA / VEGF Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C355125-200
Price: 566.0000 USD
Availability: InStock

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Antibody:

VEGFA / VEGF Mouse anti-Human Monoclonal (16F1) Antibody

Application:

IF, WB, ELISA, SwELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$566

LS-C355125-200

Toll Free North America

206-374-1102

For Research Use Only

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Overview

Antibody:

VEGFA / VEGF Mouse anti-Human Monoclonal (16F1) Antibody

Application:

IF, WB, ELISA, SwELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

VEGF antibody LS-C355125 is an unconjugated mouse monoclonal antibody to human VEGF (VEGFA). Validated for ELISA, IF, SwELISA and WB.

Target

Human VEGFA / VEGF

Synonyms

See All VEGFA / VEGF Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG,k Monoclonal

Clone

16F1

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Immunogen

Full-length human recombinant protein expressed in E-coli.

Specificity

The VEGF-A monoclonal antibody clone # 16F1 has successfully been paired as the coating antibody in a sandwich ELISA with biotinylated detection antibody LS-C355474. Typical dilutions for sandwich ELISA include 1 ug/ml for coating and 0.125-0.5 ug/ml for detection.

Applications