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Anti-VEGFA / VEGF Antibody (aa27-191, clone 26503) LS-C150096

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Wt. Vol. Conc. Price
500 µg 1000 µl 0.5 mg/ml Unavailable

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Mouse Monoclonal [clone 26503] (IgG2b) to Human VEGFA / VEGF
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human VEGFA / VEGF
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [26503]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (8 - 25 µg/ml)
  • Western blot (1 µg/ml)
  • ELISA
  • Neutralization

Specificity and Use

VEGFA / VEGF antibody was raised against s. frugiperda insect ovarian cell line Sf21-derived recombinant human VEGF165 Ala27-Arg191 Accession # NP_00 1165097.1.
aa27-191
Detects human VEGF165 and human VEGF121 in ELISAs and Western blots. In ELISAs, this antibody shows approximately 10% crossreactivity with recombinant mouse (rm) VEGF and rrVEGF and no crossreactivitywith rhVEGFD.
Western Blot, 1 µg/ml, Recombinant Human VEGF 165. under non-reducing conditions only. Immunohistochemistry, 8-25 µg/ml, Immersion fixed paraffin-embedded sections of human breast duct cancer tissue. Human VEGF Sandwich Immunoassay, Reagent. ELISA Capture, 2-8 µg/ml, Human VEGF Antibody. ELISA Detection, 0.1-0.4 µg/ml, Human VEGF 165Biotinylated Antibody. Standard, Recombinant Human VEGF 165. Neutralization, Measured by its ability to neutralize VEGF165- induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.04-0.08 µg/ml in the presence of 10 ng/ml Recombinant Human. VEGF165.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
1,000 µl Reconstitute with sterile PBS.
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About VEGFA / VEGF

P15692 NM_003376 NP_003367.4

VEGFA Antibody, VPF Antibody, Vascular permeability factor Antibody, VEGF Antibody, VEGF-A Antibody, MVCD1 Antibody

VEGFA / VEGF is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.

Neutralization

Neutralization
Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/m L) is neutralized (green line) by increasing concentrations of Human VEGF Monoclonal Antibody. The ND50 is typically 0.04-0.08 ug/ml.

Requested From: 
Date Requested: 4/25/2017

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