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Anti-VEGFA / VEGF Antibody (aa27-190) LS-C150307

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VEGFA / VEGF Antibodies

Anti-VEGFA / VEGF Antibody (clone VG76e) LS-C2929
Mouse Monoclonal [clone VG76e] (IgG1) to Human VEGFA / VEGF
Human, Bovine, Pig, Sheep
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Western blot Image
Anti-VEGFA / VEGF Antibody LS-C104581
Rabbit Polyclonal to Human VEGFA / VEGF
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-VEGFA / VEGF Antibody (clone VG-1) IHC-plus™ LS-B3579
Mouse Monoclonal [clone VG-1] (IgG1) to Human VEGFA / VEGF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Rat VEGFA / VEGF
Rat
IHC, Western blot
Unconjugated

Details

Rat VEGFA / VEGF
Goat
Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)

Specificity and Use

VEGFA / VEGF antibody was raised against mouse myeloma cell line NS0-derived recombinant rat VEGF164 Ala27-Arg190 Accession # AAL07526.
aa27-190
Detects rat VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh).
Western Blot, 0.1 µg/ml, Recombinant Rat VEGF164. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About VEGFA / VEGF

P15692 NM_003376 NP_003367.4

VEGFA Antibody, VPF Antibody, Vascular permeability factor Antibody, VEGF Antibody, VEGF-A Antibody, MVCD1 Antibody

VEGFA / VEGF is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.

Immunohistochemistry

Immunohistochemistry
VEGF164 in Rat Kidney. VEGF164 was detected in perfusion fixed frozen sections of rat kidney using 15 ug/ml Rat VEGF164 Antigen Affinity-purified Polyclonal Antibody overnight at 4 ° C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Neutralization

Neutralization
Cell Proliferation Induced by VEGF164 and Neutralization by Rat VEGF Antibody. Recombinant Rat VEGF164 stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat VEGF164 (20 ng/m L) is neutralized (green line) by increasing concentrations of Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-0.6 ug/ml.

Requested From: United States
Date Requested: 12/3/2016

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