Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
VEGF antibody was raised against e. coli-derived rcaVEGF164.
Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect canine VEGF. The detection limit for rcaVEGF is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry -This antibody will detect VEGF in cells. The working dilution is 15 µg/ml. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect canine VEGF. The detection limit for rcaVEGF is approximately 0.2 ng/well.
Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.