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Anti-VCP Antibody (clone 4G9) IHC-plus™ LS-B1912

Note: This antibody replaces LS-C40951

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular VCP Antibodies

Anti-VCP Antibody (clone 4G9) IHC-plus™ LS-B1912
Mouse Monoclonal [clone 4G9] (IgG1) to Human VCP
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (3)
Mouse Monoclonal [clone 4G9] (IgG1) to Human VCP
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Human VCP
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4G9]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1 - 4 µg/ml)
  • Western blot
  • Immunoprecipitation

Specificity and Use

VCP antibody was raised against recombinant human VCP.
Recombinant human valosin containing protein
Immunohistochemistry: LS-B1912 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1912 was determined to be 5 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced in TCS.
Store at +4°C. DO NOT FREEZE.
For research use only.

Publications (3)

1
Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis. Shchedrina VA, Everley RA, Zhang Y, Gygi SP, Hatfield DL, Gladyshev VN. The Journal of biological chemistry. 2011 286:42937-48. [PubMed:22016385] [PMC:PMC3234841]
2
Altered intracellular localization and valosin-containing protein (p97 VCP) interaction underlie ATP7A-related distal motor neuropathy. Yi L, Donsante A, Kennerson ML, Mercer JF, Garbern JY, Kaler SG. Human molecular genetics. 2012 21:1794-807. [PubMed:22210628] [PMC:PMC3313796]
3
Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells. Yahiro K, Tsutsuki H, Ogura K, Nagasawa S, Moss J, Noda M. Infection and immunity. 2012 80:1803-14. [PubMed:22354021] [PMC:PMC3347452]

About VCP

P55072 NM_007126 NP_009057.1

VCP Antibody, 15S Mg(2+)-ATPase p97 subunit Antibody, ALS14 Antibody, TER ATPase Antibody, TERA Antibody, Valosin containing protein Antibody, Yeast Cdc48p homolog Antibody, Valosin-containing protein Antibody, IBMPFD Antibody, p97 Antibody

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process.

Immunohistochemistry

Immunohistochemistry
Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1912 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI

Western blot

Western blot
Western blot of extracts from HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
 

Requested From: United States
Date Requested: 12/4/2016

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