Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Mouse Monoclonal [clone MaVinc] (IgG) to Human VCL / Vinculin
Human, Monkey, Rat, Hamster
IHC - Frozen, Immunofluorescence, Western blot
Human VCL / Vinculin
Human, Monkey, Rat, Hamster (tested or 100% immunogen sequence identity)
IgG Monoclonal [MaVinc]
IHC - Frozen (1:50 - 1:200)
Immunofluorescence (1:10 - 1:40)
Western blot (1:500 - 1:2000)
Specificity and Use
VCL / Vinculin antibody was raised against vinculin purified from chick gizzard.
Vinculin, a cytoskeletal protein involved in cell to cell and cell to matrix focal adhesions. It is highly selective for vinculin in immunofluorescent staining of cultured normal human fibroblasts in which cell sustratum focal adhesions are abundant.
Ascites, 0.09% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia.