Target
Mouse VCAM1 / CD106
Synonyms
VCAM1 | CD106 | CD106 antigen | INCAM-100 | V-CAM 1 | VCAM-1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
R. Phycoerythrin.
Also available
Unconjugated.
Purification
Protein G purified
Immunogen
Stromal cell line PA6.
Specificity
Recognizes murine vascular adhesion molecule 1 (VCAM-1), a cell surface glycoprotein that is also known as CD106. CD106 is expressed predominantly on endothelial cells and expression is up-regulated during inflammation. The ligand for CD106 is the alpha 4 subunit (CD49d) of the integrin VLA-4 (CD49d/CD29). Clone MVCAM. A(429) is reported to partially block VCAM-1 mediated functions. XXX recommend the use of MCA2297EL for this purpose.
Applications
- Flow Cytometry (1:1 - 1:10)
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store untiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee