Human VASP

Mouse

Human, Monkey, Mouse, Rat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

16C2

Unconjugated

Gel filtration

Unmodified

KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).

pSer239

Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.

• ICC
• Western blot
• Immunoprecipitation
• Flow Cytometry (2.4 µg/ml)
• ELISA (0.05 µg/ml)

Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X. FACS: 2.4 ug/ml using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 ug/ml, as reported by an independent laboratory.

Supplied as a lyophilized powder from PBS, 0.09% sodium azide, PEG, sucrose.

1 ml ddH2O

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P50552 NCBI: NM_003370 NP_003361.1