V5 Tag

Mouse

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

Paramyxovirus SV5, Simian-Virus 5.

Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.

• IHC
• IHC - Frozen
• Western blot (1:1000 - 1:5000)
• Immunoprecipitation
• ELISA (1:1000 - 1:5000)
• Radioimmunoassay

ELISA:1:1000-1:5000. Western Blot: 1:1000-1:5000. Immunoprecipitation. Radioimmunoassay. Immunohistochemistry (frozen.

0.1% Sodium Azide

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.