Protein G purified
- IHC - Frozen
- Western blot (1:1000 - 1:5000)
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V5 Tag antibody was raised against paramyxovirus SV5, Simian-Virus 5.
Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.
Western Blot:1:1000-1:5000. ELISA. Immunohistochemistry (frozen). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
IgG conjugated to Biotin. Supplied as a liquid, 0.1% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
For research use only.
This antibody carries the LSBio 100% Guarantee.