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V5 Tag
V5 Tag, GKPIPNPLLGLDST, is from the C-terminal sequence of the P and V proteins of Simian Virus 5. V5 tag antibodies are used in many applications such as western blot, immunoprecipitation and immunofluorescence, and are a reliable means of purifying target proteins in many species. The V5 tag is often used alongside His-tags in affinity purification (PMID 24510596).
V5 Tag Target Details
| Target Name: | V5 Tag |
Publications (1)
1
Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Gi Bum Kim, David Rincon Fernandez Pacheco, David Saxon, Amy Yang, Sara Sabet, Marina Dutra-Clarke, Rachelle Levy, Ashley Watkins, Hannah Park, Aslam Abbasi Akhtar, Paul W Linesch, Naomi Kobritz, Swasty S Chandra, Katie Grausam, Alberto Ayala-Sarmiento, Jessica Molina, Kristyna Sedivakova, Kendy Hoang, Jeremiah Tsyporin, Daniel S Gareau, Mariella G Filbin, Serguei Bannykh, Chintda Santiskulvong, Yizhou Wang, Jie Tang, Mario L Suva, Bin Chen, Moise Danielpour, Joshua J Breunig. Cell. 2019 September;179:251-267.e24. [Full Text Article]
[PubMed:31539496]
[PMC:PMC6934691]
Related Antibodies: LS-C136566.
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V5 Tag
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V5 Tag Sheep Polyclonal Antibody
IHC, WB
Unconjugated
500 µg/$393
V5 Tag Mouse anti-Virus Monoclonal (SV5-Pk1) Antibody
Virus
ELISA, Flo, IF, IHC-Fr, IP, RIA, WB
Unconjugated
0.1 mg/$325; 1 mg/$717; 2 mg/$1,276
V5 Tag Goat Polyclonal (aa95-108) Antibody
ELISA, ICC, IP, WB
Unconjugated
100 µg/$509
![V5 Tag Antibody - Anti-V5 Epitope Tag Polyclonal Antibody - Western Blot. Anti-V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 ug/ml to detect 0.05 ug (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/v5-tag-antibody-ls-c154221/81901_5171287.jpg)
V5 Tag Rabbit Polyclonal Antibody
ELISA, IHC, WB
Unconjugated
100 µg/$435
V5 Tag Mouse anti-Virus Monoclonal (FITC) (SV5-Pk4) Antibody
Virus
Flo, IF
FITC Conjugated
0.1 mg/$453
V5 Tag Mouse Monoclonal (4C12E11) Antibody
ELISA, Flo, IP, WB
Unconjugated
100 µg/$402; 200 µg/$559
V5 Tag Mouse Monoclonal Antibody
ELISA, IF, IP, WB
Unconjugated
100 µg/$553; 1 mg/$1,001
V5 Tag Mouse Monoclonal (PE) Antibody
ELISA, IF, IP, WB
PE Conjugated
100 µl/$832
V5 Tag Mouse anti-Virus Monoclonal (FITC) (SV5-Pk1) Antibody
Virus
IF
FITC Conjugated
0.1 mg/$453
V5 Tag Mouse Monoclonal (E10) Antibody
ELISA, IHC, WB
Unconjugated
100 µg/$316
V5 Tag Mouse anti-Virus Monoclonal (Biotin) (SV5-Pk1) Antibody
Virus
ELISA, IHC-Fr, WB
Biotin Conjugated
0.1 mg/$453
V5 Tag Mouse Monoclonal Antibody
ICC, IF, IP, WB
Unconjugated
100 µl/$267
V5 Tag Mouse Monoclonal (1H6) Antibody
ICC, IP, WB
Unconjugated
100 µg/$430
V5 Tag Rabbit Polyclonal (aa95-108) Antibody
ELISA, ICC, WB
Unconjugated
100 µg/$504
V5 Tag Rabbit Polyclonal (FITC) Antibody
ELISA, WB
FITC Conjugated
200 µl/$952
![V5 Tag Antibody - Western blot of V5-tagged fusion protein using V5-tag Antibody [HRP], pAb, Rabbit (V5-tag Antibody [HRP], pAb, Rabbit, 1 ug/ml) The signal was developed with LumiSensor HRP Substrate Kit Predicted Size: 52 kD Observed Size: 52 kD This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com/image2/v5-tag-antibody-v5-epitope-hrp-ls-c51628/128235_301643.jpg)
V5 Tag Rabbit Polyclonal (V5 epitope) (HRP) Antibody
ELISA, WB
HRP Conjugated
40 µg/$297
V5 Tag Mouse Monoclonal (Q31) Antibody
WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
V5 Tag Rabbit anti-Rabbit Polyclonal Antibody
Rabbit
ELISA, Flo, IF, Micro
Unconjugated
100 µg/$544
V5 Tag Mouse Monoclonal Antibody
ELISA, IF, IP, WB
Unconjugated
100 µg/$582; 1 mg/$1,027
V5 Tag Mouse Monoclonal (PE) Antibody
ELISA, IF, IP, WB
PE Conjugated
100 µl/$860
V5 Tag Mouse anti-Virus Monoclonal (SV5-Pk4) Antibody
Virus
ELISA, IF, IP, WB
Unconjugated
0.1 mg/$371; 1 mg/$697
V5 Tag Mouse anti-Virus Monoclonal (SV5-Pk5) Antibody
Virus
ELISA, IF, IP, WB
Unconjugated
0.1 mg/$371; 1 mg/$678
V5 Tag Mouse Monoclonal Antibody
ELISA, IHC, IHC-Fr, IP, RIA, WB
Unconjugated
100 µg/$503; 1 mg/$1,006
V5 Tag Rabbit Polyclonal (HRP) Antibody
ELISA, ICC, IHC, WB
HRP Conjugated
0.1 ml/$454
V5 Tag Rabbit Polyclonal Antibody
ELISA, ICC, IP, WB
Unconjugated
0.1 ml/$398
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