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Anti-UTF1 Antibody (aa44-153) LS-C40111

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UTF1 Antibodies

Anti-UTF1 Antibody (aa251-300) IHC-plus™ LS-B6507
Rabbit Polyclonal to Human UTF1
Human, Mouse, Rat, Guinea pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-UTF1 Antibody (aa164-199) IHC-plus™ LS-B13197
Rabbit Polyclonal to Human UTF1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human UTF1
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human UTF1
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC
  • Western blot
  • ELISA

Specificity and Use

UTF1 antibody was raised against e. coli-derived rhUTF1 (aa44-153).
aa44-153
Selected for its ability to recognize UTF1 in the applications listed.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 10 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry: This antibody can be used at a concentration of 10 ug/ml to detect UTF1 in mouse D3 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Northern Lights 557-coupled anti-donkey IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 1.0 ng/well.

Packaging

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose.
100 µl Sterile 40-50% glycerol, PBS
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid frequent freeze thaws.
For research use only.

About UTF1

Q5T230 NM_003577 NP_003568.2

UTF1 Antibody

Acts as a transcriptional coactivator of ATF2.

Requested From: United States
Date Requested: 12/9/2016

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