(tested or 100% immunogen sequence identity)
UTF1 antibody was raised against e. coli-derived rhUTF1 (aa44-153).
Selected for its ability to recognize UTF1 in the applications listed.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 10 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry: This antibody can be used at a concentration of 10 ug/ml to detect UTF1 in mouse D3 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Northern Lights 557-coupled anti-donkey IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 1.0 ng/well.
Lyophilized Sterile Solution PBS, Supplied as a Powder, 5% Trehalose
100 µl Sterile 40-50% glycerol, PBS
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
For research use only.