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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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UTF1 Antibody (aa44‑153) LS‑C40111 Best Seller
Antibody
UTF1 antibody LS-C40111 is an unconjugated goat polyclonal antibody to human UTF1. Validated for ELISA, ICC and WB.
Catalog
Size
Price
LS-C40111-100
100 µg
Unavailable

Popular UTF1 Products

UTF1 antibody (1 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-UTF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6507 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Guinea pig
Applications: IHC, IHC - Paraffin, Western blot
Anti-Utf1 Antibody at 1:2000 dilution + mouse liver lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

UTF1 antibody LS-C40111 is an unconjugated goat polyclonal antibody to human UTF1. Validated for ELISA, ICC and WB.
About UTF1
Acts as a transcriptional coactivator of ATF2. Q5T230 NM_003577 NP_003568.2

UTF1 Antibody


Specifications

Target
Human UTF1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • ELISA
Immunogen
UTF1 antibody was raised against e. coli-derived rhUTF1 (aa44-153).
Epitope
aa44-153
Specificity
Selected for its ability to recognize UTF1 in the applications listed.
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 10 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry: This antibody can be used at a concentration of 10 ug/ml to detect UTF1 in mouse D3 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Northern Lights 557-coupled anti-donkey IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 1.0 ng/well.
Presentation
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose.
Reconstitution
100 µl Sterile 40-50% glycerol, PBS
Storage
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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