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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UST Antibody (aa301‑350) LS‑C80912 Best Seller
UST antibody LS-C80912 is an unconjugated rabbit polyclonal antibody to UST from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C80912-100
100 µg
Unavailable

Popular UST Products

Antibody
Species: Human
Applications: Western blot, ELISA
UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, ELISA

Product Description

UST antibody LS-C80912 is an unconjugated rabbit polyclonal antibody to UST from human, mouse, rat and other species. Validated for IHC and WB.
About UST
Sulfotransferase that catalyzes the transfer of sulfate to the position 2 of uronyl residues. Has mainly activity toward iduronyl residues in dermatan sulfate, and weaker activity toward glucuronyl residues of chondroitin sulfate. Has no activity toward desulfated N-resulfated heparin. Q9Y2C2 NM_005715 NP_005706.1

UST Antibody, 2OST Antibody, DS2ST Antibody, Uronyl 2-sulfotransferase Antibody, Uronyl-2-sulfotransferase Antibody


Specifications

Target
Human UST
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
UST antibody was raised against synthetic peptide located between aa301-350 of human UST (Q9Y2C2, NP_005706). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Elephant, Opossum, Turkey, Zebra finch, Chicken (85%); Platypus (83%).
Blocking Peptide
LS-E16976 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80912. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa301-350
Specificity
Human UST
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 2.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UST antibody LS-C80912 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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