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Anti-USP7 / HAUSP Antibody (aa30-41) IHC-plus™ LS-B434

Note: This antibody replaces LS-C18862, LS-C93283

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular USP7 / HAUSP Antibodies

Anti-USP7 / HAUSP Antibody (aa30-41) IHC-plus™ LS-B434
Rabbit Polyclonal to Human USP7 / HAUSP
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-USP7 / HAUSP Antibody (aa1050-1100) IHC-plus™ LS-B7235
Rabbit Polyclonal to Human USP7 / HAUSP
Human, Mouse, Rat, Dog, Horse, Chicken, Macaque
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-USP7 / HAUSP Antibody (aa320-348) LS-C160480
Rabbit Polyclonal to Human USP7 / HAUSP
Human
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human USP7 / HAUSP
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human USP7 / HAUSP
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:15000 - 1:60000)

Specificity and Use

USP7 / HAUSP antibody was raised against synthetic peptide from human USP7.
aa30-41
Amino acids 30-41 of human HAUSP protein.
Immunohistochemistry: LS-B434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B434 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About USP7 / HAUSP

Q93009 NM_003470 NP_003461.2

USP7 Antibody, Deubiquitinating enzyme 7 Antibody, HAUSP Antibody, TEF1 Antibody, Ubiquitin specific protease 7 Antibody, Ubiquitin thioesterase 7 Antibody

Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-USP7 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B434 concentration 5 ug/ml.

Western blot

Western blot
Anti-HAUSP Antibody - Western Blot. Western blot of affinity purified anti-HAUSP antibody shows detection of HAUSP in various cell lysates at 130 kD (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.

Requested From: United States
Date Requested: 12/8/2016

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