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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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USP4 Antibody (clone 5E12) LS‑C198411
USP4 antibody LS-C198411 is an unconjugated mouse monoclonal antibody to human USP4. Validated for ELISA and WB.
Catalog
Size
Price
LS-C198411-100
100 µg
Unavailable

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Antibody
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Anti-USP4 antibody LS-A8558 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Applications: IHC, IHC - Paraffin, Western blot

Product Description

USP4 antibody LS-C198411 is an unconjugated mouse monoclonal antibody to human USP4. Validated for ELISA and WB.
About USP4
Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER. Q13107 NM_003363 NP_003354.2

USP4 Antibody, Deubiquitinating enzyme 4 Antibody, Unph Antibody, Ubiquitin thiolesterase 4 Antibody, Ubiquitin thioesterase 4 Antibody, Ubiquitin-specific protease 4 Antibody, UNP Antibody


Specifications

Target
Human USP4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [5E12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
USP4 antibody was raised against uSP4 (NP_003354, aa676-773 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human UNP / USP4
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.

Western blot

Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.

Western blot

Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.

Western blot

Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.


Requested From: 
Date Requested: 6/20/2018

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