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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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USP15 Antibody (aa920‑981) LS‑C185639
USP15 antibody LS-C185639 is an unconjugated rabbit polyclonal antibody to USP15 from human, bovine and chicken. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C185639-100
100 µl (0.87 mg/ml)
Unavailable

Popular USP15 Products

Anti-USP15 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6452 concentration 2.5 ug/ml.
Species: Human, Monkey, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry Image
Species: Human
Applications: IHC, Western blot, ELISA
Detection of Mouse USP15 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-USP15 used at a dilution of 1:100. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
Species: Human, Mouse, Bovine, Dog, Horse, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Immunofluorescence
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

USP15 antibody LS-C185639 is an unconjugated rabbit polyclonal antibody to USP15 from human, bovine and chicken. Validated for ICC, IHC and WB.
About USP15
Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed:21947082). Q9Y4E8 NM_006313 NP_006304.1

USP15 Antibody, Deubiquitinating enzyme 15 Antibody, KIAA0529 Antibody, Ubiquitin thioesterase 15 Antibody, Ubiquitin specific protease 15 Antibody, UNPH-2 Antibody, UNPH4 Antibody, Ubiquitin thiolesterase 15 Antibody


Specifications

Target
Human USP15
Host
Rabbit
Reactivity
Human, Bovine, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
USP15 antibody was raised against synthetic peptide corresponding to a region within amino acids 920 and 981 of USP15 (SwissProt Q9Y4E8). Percent identity by BLAST analysis: Human, Chicken, Bovine (100%).
Epitope
aa920-981
Specificity
Human USP15
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using USP15 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using USP15 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1. 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. USP15 antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/24/2018

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