Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Rabbit Polyclonal (IgG) to Human USP13
Human, Mouse (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
Western blot (1:500 - 1:3000)
Specificity and Use
USP13 antibody was raised against recombinant fragment corresponding to a region within amino acids 597 and 817 of USP13 (SwissProt Q92995). Percent identity by BLAST analysis: Human (100%); Bovine (96%); Rat (88%).
Human, mouse USP13
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability.