LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-US01 / p115 Antibody (Internal) LS-C95265

Ordering

Wt. Vol. Conc. Price
500 µg 500 µl 1 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular US01 / p115 Antibodies

Anti-US01 / p115 Antibody (Internal) LS-C95265
Rabbit Polyclonal (IgG) to Human US01 / p115
Human, Mouse, Rat
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-US01 / p115 Antibody (clone 3F4) IHC-plus™ LS-B6185
Mouse Monoclonal [clone 3F4] (IgG1,k) to Human US01 / p115
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-US01 / p115 Antibody (aa913-962) IHC-plus™ LS-B11369
Rabbit Polyclonal (IgG) to Human US01 / p115
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human US01 / p115
Human, Mouse, Rat
Immunofluorescence, Western blot
Unconjugated

Details

Human US01 / p115
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • Immunofluorescence
  • Western blot (10 - 50 µg/ml)

Specificity and Use

US01 / p115 antibody was raised against synthetic peptide from the internal part of human Vesicle-docking protein (VDP, USO1, TAP, Transcytosis-associated protein) conjugated to an immunogenic carrier protein. The antigen is homologous in rat and moue.
Internal
Specific for VDP.
IHC: Antigen retrieval is essential for use on paraffin sections.

Packaging

Lyophilized. Centrifuge to remove any insoluble material
500 µl Sterile water
Maintain lyophilized and reconstituted antibodies at -20 °C for long term storage and at 2-8 °C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
For research use only.

About US01 / p115

O60763 NM_003715 NP_003706.2

USO1 Antibody, p115 Antibody, Protein USO1 homolog Antibody, TAP Antibody, Vesicle docking protein p115 Antibody, Vesicle-docking protein Antibody, US01 Antibody, USO1 vesicle transport factor Antibody, VDP Antibody

US01 / p115 is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation.

Immunofluorescence

Immunofluorescence
Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Immunofluorescence

Immunofluorescence
Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Immunofluorescence

Immunofluorescence
Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Immunofluorescence

Immunofluorescence
Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Requested From: United States
Date Requested: 12/5/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number