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Antibodies > US01 / p115 > LS-C95265
 
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Catalog Number Size Price
LS-C95265-500 500 µg $393 
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
US01 / p115 Antibody - Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
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Polyclonal Rabbit anti‑Human US01 / p115 Antibody (Internal, IF, WB) LS‑C95265

Polyclonal Rabbit anti‑Human US01 / p115 Antibody (Internal, IF, WB) LS‑C95265

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Antibody:
US01 / p115 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C95265-500
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
US01 / p115 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
P115 antibody LS-C95265 is an unconjugated rabbit polyclonal antibody to p115 (US01) (Internal) from human. It is reactive with human, mouse and rat. Validated for IF and WB.
Target
Human US01 / p115
Synonyms
USO1 | p115 | Protein USO1 homolog | TAP | Vesicle docking protein p115 | Vesicle-docking protein | US01 | USO1 vesicle transport factor | VDP
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the internal part of human Vesicle-docking protein (VDP, USO1, TAP, Transcytosis-associated protein) conjugated to an immunogenic carrier protein. The antigen is homologous in rat and moue.
Epitope
Internal
Specificity
Specific for VDP.
Applications
  • Immunofluorescence
  • Western blot (10 - 50 µg/ml)
Usage
IHC: Antigen retrieval is essential for use on paraffin sections.
Presentation
Lyophilized
Reconstitution
500 µl Sterile water
Storage
Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About US01 / p115
O60763 NM_003715 NP_003706.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/15/2024
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