(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- Western blot (1:1000)
- ELISA (1:2000 - 1:10000)
URM1 antibody was raised against recombinant yeast Urm1 protein.
This purified polyclonal antibody reacts with yeast Urm1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. A 12 kD band corresponding to yeast Urm1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Lyophilized 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
0.1 ml ddH2O
Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
For research use only.