LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
UPF3B Antibody (aa252‑301) LS‑C101886
UPF3B antibody LS-C101886 is an unconjugated rabbit polyclonal antibody to UPF3B from human, dog, monkey and other species. Validated for WB.
Catalog
Size
Price
LS-C101886-50
50 µg
Unavailable

Popular UPF3B Products

UPF3B antibody LS-C31657 was used in IHC to stain formalin-fixed, paraffin-embedded human stomach.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunoprecipitation
UPF3B/RENT3B antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
Species: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry - Paraffin Image
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

UPF3B antibody LS-C101886 is an unconjugated rabbit polyclonal antibody to UPF3B from human, dog, monkey and other species. Validated for WB.
About UPF3B
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. Q9BZI7 NM_023010 NP_075386.1

UPF3B Antibody, HUPF3B Antibody, MRXS14 Antibody, HUpf3p-X Antibody, RENT3B Antibody, UPF3X Antibody


Specifications

Target
Human UPF3B
Host
Rabbit
Reactivity
Human, Monkey, Dog, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
UPF3B antibody was raised against synthetic peptide located between aa252-301 of human UPF3B (Q9BZI7, NP_542199). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Rabbit (100%); Mouse, Rat (92%).
Blocking Peptide
LS-E15083 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101886. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa252-301
Specificity
Human UPF3B
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number