Target
Human UPF3A / UPF3
Synonyms
UPF3A | HUPF3A | HUpf3 | RENT3A | UPF3
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KETDKQKKIAEKE, from the internal region of the protein sequence according to NP_075387.1NP_542418.1.
Specificity
Human UPF3A. This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1).
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51kDa according to NP_542418.1 and the reported sizes of 65 to 70kDa from different sources. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee