Target
Human ULK3
Synonyms
ULK3 | Unc-51-like kinase 3 | DKFZP434C131
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KMRESRWEADTLDK, from the C-Terminus of protein sequence according to NP_001092906.1.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kD band observed in Human Brain (Cerebellum, Cerebral Cortex, Hippocampus) lysates (calculated MW of 53.4kD according to NP_001092906.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 75kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee