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Anti-ULK1 Antibody IHC-plus™ LS-B1811

Note: This antibody replaces LS-C47392

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ULK1 Antibodies

Anti-ULK1 Antibody LS-C167359
Rabbit Polyclonal to Human ULK1
Human, Mouse
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-ULK1 Antibody (Internal) IHC-plus™ LS-B10104
Rabbit Polyclonal (IgG) to Human ULK1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ULK1
Human, Monkey
IHC - Paraffin, Western blot
Unconjugated

Details

Human ULK1
Rabbit
Human, Monkey (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

ULK1 antibody was raised against a portion of amino acid320-370of human ULK1.
A portion of amino acid 320-370 of human ULK1.
Immunohistochemistry: LS-B1811 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1811 was determined to be 10 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About ULK1

O75385 NM_003565 NP_003556.1

ULK1 Antibody, ATG1A Antibody, ATG1 Antibody, HATG1 Antibody, KIAA0722 Antibody, Unc-51-like kinase 1 Antibody, Unc51.1 Antibody, UNC51 Antibody

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR.

Immunohistochemistry

Immunohistochemistry
Anti-ULK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1811 concentration 10 ug/ml.

Western blot

Western blot
Western blot of ULK1 in Raji cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using antibody at 2 ug/ml.

Requested From: United States
Date Requested: 12/10/2016

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