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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UIMC1 / RAP80 Antibody (N‑Terminus) IHC‑plus™ LS‑B2707
Note: This antibody replaces LS-C34742
RAP80 antibody LS-B2707 is an unconjugated rabbit polyclonal antibody to RAP80 (UIMC1) from human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2707-50
50 µg (1 mg/ml)
Unavailable

Popular UIMC1 / RAP80 Products

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Species: Human, Mouse
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Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.
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Detection of Human RAP80 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAP80 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
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Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

RAP80 antibody LS-B2707 is an unconjugated rabbit polyclonal antibody to RAP80 (UIMC1) from human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About UIMC1 / RAP80
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Q96RL1 NM_016290 NP_057374.3

UIMC1 Antibody, BRCA1-A complex subunit RAP80 Antibody, Receptor associated protein 80 Antibody, RAP80 Antibody, RXRIP110 Antibody, X2HRIP110 Antibody, Receptor-associated protein 80 Antibody


Specifications

Target
Human UIMC1 / RAP80
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30388 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B2707. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Usage
Immunohistochemistry: LS-B2707 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2707 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.

Western blot

Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.
Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.

Western blot

Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.
Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.

Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.

Western blot

Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.
Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.

Western blot

Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.
Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.

Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.

Western blot

Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.
Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.

Western blot

Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.
Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.

Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.
Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/ml.

Western blot

Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.
Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 µg/mL.

Western blot

Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.
Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 µg/ml.


Requested From: 
Date Requested: 6/20/2018

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