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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UIMC1 / RAP80 Antibody (aa535‑548) IHC‑plus™ LS‑B7372
Note: This antibody replaces LS-C112668
RAP80 antibody LS-B7372 is an unconjugated goat polyclonal antibody to RAP80 (UIMC1) from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7372-50
50 µg (0.5 mg/ml)
Unavailable

Popular UIMC1 / RAP80 Products

Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2706 concentration 5 ug/ml.
Species: Human, Mouse
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Anti-UIMC1 / RAP80 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2707 concentration 5 ug/ml.
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Detection of Human RAP80 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAP80 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
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Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

RAP80 antibody LS-B7372 is an unconjugated goat polyclonal antibody to RAP80 (UIMC1) from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About UIMC1 / RAP80
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Q96RL1 NM_016290 NP_057374.3

UIMC1 Antibody, BRCA1-A complex subunit RAP80 Antibody, Receptor associated protein 80 Antibody, RAP80 Antibody, RXRIP110 Antibody, X2HRIP110 Antibody, Receptor-associated protein 80 Antibody


Specifications

Target
Human UIMC1 / RAP80
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Dog (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
UIMC1 / RAP80 antibody was raised against synthetic peptide C-TRRQKEAKTKSDSG from an internal region of human UIMC1 / RAP80 (NP_057374.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog (93%); Rabbit, Pig (86%).
Blocking Peptide
LS-E27141 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B7372. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa535-548
Specificity
Human UIMC1 / RAP80.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 60 kDa band in Human Heart and Spleen lysates and in lysates of cell lines A431, HeLa and HepG2 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.7 kDa according to NP_057374.3. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.
Anti-UIMC1 / RAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7372 concentration 3.75 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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