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Anti-UHRF1 Antibody (aa229-257) LS-C160423

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UHRF1 Antibodies

Anti-UHRF1 Antibody (clone 3A11) LS-C105136
Mouse Monoclonal [clone 3A11] (IgG1,k) to Human UHRF1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image
Anti-UHRF1 Antibody (clone Th-10a) LS-C179534
Rat Monoclonal [clone Th-10a] (IgG2a) to Mouse UHRF1
Mouse
ICC, Western blot
Unconjugated
Western blot Image
Anti-UHRF1 Antibody (aa584-633) IHC-plus™ LS-B14002
Rabbit Polyclonal to Human UHRF1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human UHRF1
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human UHRF1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

UHRF1 antibody was raised against kLH-conjugated synthetic peptide between amino acids 229-257 from internal region of human UHRF1.
LS-E9813 - Lyophilized - 100 µg - $145.00
aa229-257
Human UHRF1

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About UHRF1

Q96T88 NM_013282 NP_037414.3

UHRF1 Antibody, HUHRF1 Antibody, HuNp95 Antibody, Nuclear protein 95 Antibody, RING finger protein 106 Antibody, RNF106 Antibody, Transcription factor ICBP90 Antibody, HNP95 Antibody, ICBP90 Antibody, Np95 Antibody

Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with UHRF1 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UHRF1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). UHRF1 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
UHRF1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The UHRF1 antibody detected the UHRF1 protein (arrow).

Flow Cytometry

Flow Cytometry
UHRF1 Antibody flow cytometry of HeLa cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: United States
Date Requested: 12/10/2016

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