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Anti-UEV1 / UEV1A Antibody (aa31-49) IHC-plus™ LS-B2092

Note: This antibody replaces LS-C839, LS-C93225

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UEV1 / UEV1A Antibodies

Anti-UEV1 / UEV1A Antibody (C-Terminus) LS-C451
Rabbit Polyclonal to Human UEV1 / UEV1A
Human, Mouse, Rat
Western blot
Unconjugated
Western blot Image
Anti-UEV1 / UEV1A Antibody (aa31-49) IHC-plus™ LS-B2092
Rabbit Polyclonal (IgG) to Human UEV1 / UEV1A
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-UEV1 / UEV1A Antibody IHC-plus™ LS-B13894
Rabbit Polyclonal (IgG) to Human UEV1 / UEV1A
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human UEV1 / UEV1A
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human UEV1 / UEV1A
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

UEV1 / UEV1A antibody was raised against synthetic peptide from human UBE2V1.
aa31-49
A protein band of approximate molecular weight of 20 kD can be seen in Jurkat, RAW and HeLa cell lysates. This may also recognize isoform b of UBE2V1.
Immunohistochemistry: LS-B2092 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2092 was determined to be 5 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About UEV1 / UEV1A

Q13404 NM_021988 NP_068823.2

UBE2V1 Antibody, CROC1 Antibody, UEV1 Antibody, UBE2V Antibody, UEV-1 Antibody, UEV1A Antibody, CROC-1 Antibody

Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity. There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway.

Immunohistochemistry

Immunohistochemistry
Anti-UBE2V1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2092 concentration 5 ug/ml.

Western blot

Western blot
Western blot of UBE2V1a in Jurkat cell lysate using antibody at 1 ug/ml.

Requested From: United States
Date Requested: 12/4/2016

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