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Catalog Number Size Price
LS-B3249-50 50 µg (0.5 mg/ml) $485 
UCP2 Antibody - Anti-UCP2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
UCP2 Antibody - Anti-UCP2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
UCP2 Antibody - Anti-UCP2 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
UCP2 Antibody - Uncoupling protein 2 / UCP2 antibody immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
UCP2 Antibody - Uncoupling protein 2 / UCP2 antibody (1ug/ml) staining of Rat Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
UCP2 Antibody - Antibody (0.5 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
UCP2 Antibody - Anti-UCP2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
UCP2 Antibody - Anti-UCP2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
UCP2 Antibody - Anti-UCP2 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
UCP2 Antibody - Uncoupling protein 2 / UCP2 antibody immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
UCP2 Antibody - Uncoupling protein 2 / UCP2 antibody (1ug/ml) staining of Rat Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
UCP2 Antibody - Antibody (0.5 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human UCP2 Antibody (aa101‑113, IHC, IF, WB) LS‑B3249

IHC‑plus™ Polyclonal Goat anti‑Human UCP2 Antibody (aa101‑113, IHC, IF, WB) LS‑B3249

Note: This antibody replaces LS-C55648
Antibody:
UCP2 Goat anti-Human Polyclonal (aa101-113) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B3249-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
UCP2 Goat anti-Human Polyclonal (aa101-113) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
UCP2 antibody LS-B3249 is an unconjugated goat polyclonal antibody to UCP2 (aa101-113) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human UCP2
Synonyms
UCP2 | BMIQ4 | UCP 2 | Uncoupling protein 2 | UCPH | SLC25A8
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DSVKQFYTKGSEH, from the internal region of the protein sequence according to NP_003346.2.
Epitope
aa101-113
Specificity
Human UCP2.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3249 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3249 was determined to be 3.75 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UCP2
P55851 NM_003355 NP_003346.2

Validation

UCP2 Antibody - Anti-UCP2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-UCP2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-UCP2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

UCP2 Antibody - Anti-UCP2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-UCP2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-UCP2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

UCP2 Antibody - Anti-UCP2 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-UCP2 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-UCP2 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

LSBio Ratings

IHC-plus™ UCP2 Antibody (aa101-113) for IHC, IF/Immunofluorescence, WB/Western LS-B3249 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)

Publications (4)

Defective mitophagy in XPA via PARP-1 hyperactivation and NAD(+)/SIRT1 reduction. Fang EF, Scheibye-Knudsen M, Brace LE, Kassahun H, SenGupta T, Nilsen H, Mitchell JR, Croteau DL, Bohr VA. Cell. 2014 May;157:882-96.

AKI after conditional and kidney-specific knockdown of stanniocalcin-1. Huang L, Belousova T, Pan JS, Du J, Ju H, Lu L, Zhang P, Truong LD, Nuotio-Antar A, Sheikh-Hamad D. Journal of the American Society of Nephrology : JASN. 2014 25:2303-15. (IHC, WB; Mouse)

Overexpression of stanniocalcin-1 inhibits reactive oxygen species and renal ischemia/reperfusion injury in mice. Huang L, Belousova T, Chen M, DiMattia G, Liu D, Sheikh-Hamad D. Kidney international. 2012 82:867-77. (WB; Human)

Human stanniocalcin-1 suppresses angiotensin II-induced superoxide generation in cardiomyocytes through UCP3-mediated anti-oxidant pathway. Dajun Liu, Luping Huang, Yanlin Wang, Wei Wang, Xander H T Wehrens, Tatiana Belousova, Maen Abdelrahim, Gabriel DiMattia, David Sheikh-Hamad. PloS one. 2012 June;7:e36994.

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Requested From: United States
Date Requested: 4/19/2024