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Anti-UCHL3 Antibody (Internal) IHC-plus™ LS-A8555

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
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LSBio (Direct) LSBio (Direct)
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Rabbit Polyclonal to Human UCHL3
Human, Monkey, Bovine, Dog, Horse
IHC - Paraffin
Unconjugated

Details

Human UCHL3
Rabbit
Human, Monkey, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Mouse, Rat, Pig, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (10 µg/ml)

Specificity and Use

UCHL3 antibody was raised against synthetic 18 amino acid peptide from internal region of human UCHL3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bovine, Elephant, Panda, Horse (100%); Mouse, Rat, Rabbit, Pig (94%); Opossum (83%).
LS-E29434 - Liquid - 50 µg - $145.00
Internal
Human UCHL3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A8555 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A8555 was determined to be 10 ug/ml.

Packaging

PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
For research use only.

About UCHL3

P15374 NM_006002 NP_005993.1

UCHL3 Antibody, Ubiquitin thiolesterase Antibody, Ubiquitin thioesterase L3 Antibody, UCH-L3 Antibody

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling.

Immunohistochemistry

Immunohistochemistry
Anti-UCHL3 antibody LS-A8555 IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
 

Requested From: United States
Date Requested: 9/25/2016

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