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Anti-UCHL1 / PGP9.5 Antibody IHC-plus™ LS-B3043

Note: This antibody replaces LS-C49161


Wt. Vol. Conc. Price
- 100 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular UCHL1 / PGP9.5 Antibodies

Anti-UCHL1 / PGP9.5 Antibody LS-C122356
Rabbit Polyclonal (IgG) to Human UCHL1 / PGP9.5
Human, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Anti-UCHL1 / PGP9.5 Antibody (aa171-220) IHC-plus™ LS-B5981
Rabbit Polyclonal (IgG) to Human UCHL1 / PGP9.5
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal to Human UCHL1 / PGP9.5
Human, Rat
IHC - Paraffin, Immunofluorescence, Western blot


Human UCHL1 / PGP9.5
Human, Rat (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation


  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)

Specificity and Use

UCHL1 / PGP9.5 antibody was raised against recombinant full length human UCHL1 purified from E. coli.
UCHL1 - Peripheral Nerve Fiber Marker.
Immunohistochemistry: LS-B3043 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3043 was determined to be 1:100.


Affinity purified serum, 10 mM sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About UCHL1 / PGP9.5

P09936 NM_004181 NP_004172.2

UCHL1 Antibody, Ubiquitin C-terminal hydrolase Antibody, PARK5 Antibody, PGP9.5 Antibody, PGP95 Antibody, Ubiquitin thioesterase L1 Antibody, Neuron cytoplasmic protein 9.5 Antibody, PGP 9.5 Antibody, Uch-L1 Antibody

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.


Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3043 dilution 1:100.


Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3043 dilution 1:100.


Shows rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.

Western blot

Western blot
Blots of whole cell homogenate of the human SH-SY5Y neuroblastoma cell line stained with chicken antibody to glyceraldehyde 3 phosphate dehydrogenase, GAPDH (left lane), and LS-B3043(right lane). The GAPDH antibody stains a ~37kD band while the UCHL1 antibody stains a band at about 24kD.

Requested From: United States
Date Requested: 10/24/2016

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