Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3D9] (IgG2a) to Human UCHL1 / PGP9.5
IHC, Western blot, ELISA
Human UCHL1 / PGP9.5
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [3D9]
Protein G purified
Western blot (1:1000 - 1:2000)
IHC - Paraffin
Specificity and Use
UCHL1 / PGP9.5 antibody was raised against recombinant human PGP9.5 (1-223aa) purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:2000.
PBS, pH 7.4, 0.1% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
The extracts of mouse brain (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PGP9.5(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.