Human UBQLN1 / Ubiquilin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

3D5E2

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Recombinant protein derived from an internal region of human ubiquilin

Internal

Specific for the ubiquilin protein. On Western blots, it identifies a band at ~66kD. Reactivity has been confirmed with human HEK293 cell lysates.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1 µg/ml)
• Immunoprecipitation
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B3142 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3142 was determined to be 10 ug/ml. Immunoprecipitation: 2 ug/reaction. Use native protein.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UMX0 NCBI: NM_013438 Q9UMX0