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Anti-UBP43 / USP18 Antibody (aa40-49) IHC-plus™ LS-B1182

Note: This antibody replaces LS-C19087, LS-C91868


Wt. Vol. Conc. Price
50 µg - 0.93 mg/ml Unavailable

Most Popular UBP43 / USP18 Antibodies

Anti-UBP43 / USP18 Antibody (aa40-49) IHC-plus™ LS-B1182
Rabbit Polyclonal to Human UBP43 / USP18
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-UBP43 / USP18 Antibody (aa110-159) LS-C80869
Rabbit Polyclonal (IgG) to Human UBP43 / USP18
Human, Horse, Pig
Western blot
Western blot Image
Anti-UBP43 / USP18 Antibody LS-C132833
Rabbit Monoclonal (IgG) to Human UBP43 / USP18
Western blot, Immunoprecipitation

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human UBP43 / USP18
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human UBP43 / USP18
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

UBP43 / USP18 antibody was raised against synthetic peptide from human USP18.
Amino acids 40 to 49 of human USP18
Immunohistochemistry: LS-B1182 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1182 was determined to be 5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (2)

Apigenin inhibits hepatoma cell growth through alteration of gene expression patterns. Cai J, Zhao XL, Liu AW, Nian H, Zhang SH. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2011 18:366-73. (WB; Human) [PubMed:20850954]
Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia. AbdulMajeed AA, Dalley AJ, Farah CS. Annals of diagnostic pathology. 2013 Aug;17:331-40. [Full Text Article] [PubMed:23643910]

About UBP43 / USP18

Q9UMW8 NM_017414 NP_059110.2

USP18 Antibody, 43 kDa ISG15-specific protease Antibody, ISG43 Antibody, HUBP43 Antibody, ISG15 specific protease Antibody, Ubiquitin specific protease 18 Antibody, Ubl thioesterase 18 Antibody, Ubl thiolesterase 18 Antibody, UBP43 Antibody

UBP43 / USP18 belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease.


Anti-USP18 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1182 concentration 5 ug/ml.

Western blot

Western blot
Anti-UBP43 Antibody - Western Blot. Western blot of Affinity Purified anti-UBP43 antibody shows detection of a band ~43 kD band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.

Requested From: 
Date Requested: 3/24/2017

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