Ubiquitin

Rabbit

IgG Polyclonal

Unconjugated

Ion exchange chromatography

Unmodified

Ubiquitin coupled to rabbit IgG using glutaraldehyde.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

• Western blot (1:200 - 1:1000)
• ELISA (1:1000 - 1:5000)

The detection of ubiquitinated species is further complicated by the fact that the process of ubiquitination is reversible and is accomplished by highly specific proteases present in all eukaryotic cells. It is therefore recommended that cell lysates are kept on ice and the lysis buffers contain one or more of cysteine protease inhibitors (n-ethylmaleimide, E64, etc.). Applications: Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:5000. Western Blot: 1:200-1:1000.

Lyophilized from PBS, pH 7.2, 0.1% Sodium Azide

Sterile, distilled, deionized water or sterile 40-50% glycerol

For research use only. Intended for use by laboratory professionals.

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