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Anti-Ubiquitin Antibody LS-C59541

Ordering

Wt. Vol. Conc. Price
500 µg 0.1 ml - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Ubiquitin Antibodies

Anti-Ubiquitin Antibody (clone P4D1, Biotin) LS-C106167
Mouse Monoclonal [clone P4D1] (IgG1) to Human Ubiquitin
Human
Western blot
Biotin Conjugated
Western blot Image
Anti-Ubiquitin Antibody (clone P4D1) LS-C107107
Mouse Monoclonal [clone P4D1] (IgG1)
Western blot, Immunoprecipitation
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal
Western blot, ELISA
Unconjugated

Details

Ubiquitin
Rabbit
Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:200 - 1:1000)
  • ELISA (1:1000 - 1:5000)

Specificity and Use

Ubiquitin antibody was raised against ubiquitin coupled to rabbit IgG using glutaraldehyde.
anti-Rabbit Serum
This purified polyclonal antibody reacts with ubiquitin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For detection of free Ub by western blotting use Tris-Tricine SDS-PAGE and autoclaved nitrocellulose filters after the transfer and before blocking and addition of anti-Ub antibodies. Details on western blotting procedures are found in Mimnaugh et al., (1999 and 2002).

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at 4°C.
For research use only.

About Ubiquitin

Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein.

Western blot

Western blot
Anti-Ubiquitin Antibody - Western Blot. Anti-Ubiquitin antibody, generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by western blot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # LS-C60943) for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/6/2016

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