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Anti-Ubiquitin Antibody IHC-plus™ LS-B3328

Note: This antibody replaces LS-C76676

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Ubiquitin Antibodies

Anti-Ubiquitin Antibody (clone P4D1, Biotin) LS-C106167
Mouse Monoclonal [clone P4D1] (IgG1) to Human Ubiquitin
Human
Western blot
Biotin Conjugated
Western blot Image
Anti-Ubiquitin Antibody (clone P4D1) LS-C107107
Mouse Monoclonal [clone P4D1] (IgG1)
Western blot, Immunoprecipitation
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1,k) to Bovine Ubiquitin
Bovine, Human, Mouse
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Bovine Ubiquitin
Mouse
Bovine, Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Western blot
  • ELISA (0.1 - 1 µg/ml)

Specificity and Use

Ubiquitin antibody was raised against purified bovine ubiquitin conjugated to carrier protein.
Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.
Immunohistochemistry: LS-B3328 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3328 was determined to be 10 ug/ml. Western Blot: 1-3 ug/ml; Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl, pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About Ubiquitin

Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein.

Immunohistochemistry

Immunohistochemistry
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3328 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Ubiquitin antibody IHC of human brain, cortex, senile plaque. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3328 concentration 10 ug/ml.

Requested From: United States
Date Requested: 12/7/2016

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