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Anti-Ubiquitin Antibody (clone Ubi-1-AP) LS-C204828


Wt. Vol. Conc. Price
- 100 µl 1 mg/ml Unavailable

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Mouse Monoclonal [clone Ubi-1-AP] (IgG1,k) to Mammal Ubiquitin
Mammal, Human
IHC, Immunofluorescence, Western blot


Mammal Ubiquitin
Mammal, Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [Ubi-1-AP]
Affinity purified


  • IHC (1:1000)
  • Immunofluorescence (1:500)
  • Western blot

Specificity and Use

Ubiquitin antibody was raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. MCA-Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically.
Clone Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that clone Ubi-1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. The antibody also works on paraffin embedded material. clone Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. clone Ubi-1 will recognize human, bovine, chicken, Drosophila, and C. elegans in ELISA. clone Ubi-1 also works on Western blots and can be used to study ubiquitinated proteins. It has also been successfully used in competitive ELISA.
Ubi-1 is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. We recommend a starting dilution of 1:1000 for this purposes, using ABC or other enzymatic amplification procedures. For immunofluorescence try a dilution of 1:500.


+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Ubiquitin

Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein.


MCA-Ubi-1 staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.

Western blot

Western blot
Immunoblotting of MCA-Ubi-1 on (lane 1) poly-ubiquitin (lys63 linked), (lane 2) pure ubiquitin and lanes 3-5 crude homogenates of adult rat cortex, cerebellum and brain stem.

Requested From: 
Date Requested: 3/28/2017

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