Mammal Ubiquitin

Mouse

Mammal, Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Ubi-1-AP

Unconjugated

Affinity purified

Unmodified

Purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. MCA-Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically.

Clone Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that clone Ubi-1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. The antibody also works on paraffin embedded material. clone Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. clone Ubi-1 will recognize human, bovine, chicken, Drosophila, and C. elegans in ELISA. clone Ubi-1 also works on Western blots and can be used to study ubiquitinated proteins. It has also been successfully used in competitive ELISA.

• IHC (1:1000)
• Immunofluorescence (1:500)
• Western blot

Ubi-1 is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. We recommend a starting dilution of 1:1000 for this purposes, using ABC or other enzymatic amplification procedures. For immunofluorescence try a dilution of 1:500.

Liquid

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.