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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Ubiquitin Antibody (clone 5B9‑B3) IHC‑plus™ LS‑B8143
Note: This antibody replaces LS-C108984
Ubiquitin antibody LS-B8143 is an unconjugated mouse monoclonal antibody to Ubiquitin from human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8143-50
50 µg
Unavailable

Popular Ubiquitin Products

Staining of human hypophysis. Formalin-fixed, paraffin processed tissue with Mouse anti-Ubiquitin (LS-C43546).
Species: Bovine, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Formalin-fixed, paraffin-embedded human brain Alzheimer's disease stained with peroxidase-conjugate and AEC. Note staining of senile plaques and neurons.
Species: Human
Applications: IHC, IHC - Paraffin
293T cells were treated with vehicle (1) or proteosome inhibitor (2) for 4 hours. Lysates were then immunoblotted with antibody anti-ubiquitin monoclonal antibody at 2 ug/ml and revealed with anti-mouse HRP.
Species:
Applications: Western blot, Immunoprecipitation
Anti-Ubiquitin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3695 concentration 10 ug/ml.
Species: Bovine, Human, Mouse, Rat, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-Ub antibody at 1:500 dilution. 293HEK cells transfected lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution;
Species: Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Donkey
Applications: Western blot

Product Description

Ubiquitin antibody LS-B8143 is an unconjugated mouse monoclonal antibody to Ubiquitin from human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About Ubiquitin
Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It was discovered in 1975 by Goldstein and further characterized throughout the 1970s and 1980s. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52 and RPS27A. Ubiquitination is a post-translational modification (an addition to a protein after it has been made) where ubiquitin is attached to a substrate protein.

Specifications

Target
Bovine Ubiquitin
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [5B9-B3]
Conjugations
Unconjugated. Also available conjugated with Atto 390, Atto 565, Atto 488, Atto 594, Atto 633, Atto 655, Atto 680, Atto 700, AP, APC, Biotin, FITC, PE, Atto 594, PerCP, RPE, SA.
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Ubiquitin antibody was raised against native bovine ubiquitin, conjugated to KLH.
Specificity
Recognizes ~10kD kD corresponding to free ubiquitin.
Presentation
PBS, pH 7.4, 0.09% sodium azide, 50% glycerol
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Ubiquitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8143 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Ubiquitin (5B9-B3), in human cell lysates using a 1:1000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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