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Anti-UBE2M / UBC12 Antibody (aa169-180) IHC-plus™ LS-B432

Note: This antibody replaces LS-C19091


Wt. Vol. Conc. Price
50 µg - 0.38 mg/ml $395

LSBio (Direct) LSBio (Direct)

UBE2M / UBC12 Antibody LS-B432 Images

[click image for a larger image and more details]
Anti-UBE2M antibody IHC of human kidney.

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Rabbit Polyclonal to Human UBE2M / UBC12
Human, Mouse, Rat, Dog, Frog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human UBE2M / UBC12
Human, Mouse, Rat, Dog, Frog (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (2.5 µg/ml), Immunofluorescence, Western blot (1:500 - 1:2000), ELISA (1:2000 - 1:10000)

Specificity and Use

UBE2M / UBC12 antibody was raised against synthetic peptide from human UBE2M.
aa 169-180 of Human UBC12 protein.
Immunohistochemistry: LS-B432 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B432 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About UBE2M / UBC12

P61081 NM_003969 NP_003960.1

UBE2M Antibody, NEDD8 protein ligase Antibody, NEDD8 carrier protein Antibody, NEDD8-conjugating enzyme Ubc12 Antibody, HUbc12 Antibody, UBC-RS2 Antibody, UBC12 Antibody, Ubiquitin-protein ligase M Antibody, Yeast UBC12 homolog Antibody, Ubiquitin carrier protein M Antibody

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.


Anti-UBE2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B432 concentration 5 ug/ml.

Requested From: United States
Date Requested: 10/6/2015

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