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Anti-UBE2L6 Antibody (aa1-152, clone K1H3) IHC-plus™ LS-B2964

Note: This antibody replaces LS-C93812

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBE2L6 Antibodies

Anti-UBE2L6 Antibody (aa1-152, clone K1H3) IHC-plus™ LS-B2964
Mouse Monoclonal [clone K1H3] (IgG2b,k) to Human UBE2L6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-UBE2L6 Antibody (aa124-153, FITC) LS-C272912
Rabbit Polyclonal (IgG) to Human UBE2L6
Human
IHC, Western blot, ELISA
FITC Conjugated
Anti-UBE2L6 Antibody (aa124-153, PE) LS-C272915
Rabbit Polyclonal (IgG) to Human UBE2L6
Human
IHC, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone K1H3] (IgG2b,k) to Human UBE2L6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human UBE2L6
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [K1H3]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:3000)
  • ELISA

Specificity and Use

UBE2L6 antibody was raised against recombinant human UBE2L6 (1-152aa) purified from E. coli. NCBI Accession No.: NP_004214.
aa1-152
Immunohistochemistry: LS-B2964 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2964 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About UBE2L6

O14933 NM_004223 NP_004214.1

UBE2L6 Antibody, Ubiquitin carrier protein L6 Antibody, Ubiquitin-protein ligase L6 Antibody, UBCH8 Antibody, RIG-B Antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene.

Immunohistochemistry

Immunohistochemistry
Anti-UBE2L6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2964 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-UBE2L6 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2964 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-UBE2L6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2964 concentration 5 ug/ml.

Western blot

Western blot
Cell lysates of MCF7 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: United States
Date Requested: 12/3/2016

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