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Anti-UBE2J1 Antibody (N-Terminus) LS-C60103

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBE2J1 Antibodies

Anti-UBE2J1 Antibody (aa1-30, APC) LS-C242356
Rabbit Polyclonal (IgG) to Human UBE2J1
Human
Western blot, ELISA
APC Conjugated
Anti-UBE2J1 Antibody (aa1-30, AP) LS-C242357
Rabbit Polyclonal (IgG) to Human UBE2J1
Human
Western blot, ELISA
AP Conjugated
Anti-UBE2J1 Antibody (aa1-30, FITC) LS-C242359
Rabbit Polyclonal (IgG) to Human UBE2J1
Human
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human UBE2J1
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human UBE2J1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:200 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:1000 - 1:5000)

Specificity and Use

UBE2J1 antibody was raised against synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.
N-Terminus
human Ube2j1 protein. A BLAST analysis was used to suggest cross-reactivity with Ube2j1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologs from other sources is not known
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35-40 kD in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract. Immunoprecipitation: 1-2 ug.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About UBE2J1

NM_016021 NP_057105.2

UBE2J1 Antibody, HSPC153 Antibody, HSUBC6e Antibody, HSU93243 Antibody, NCUBE1 Antibody, HSPC205 Antibody, Ubc6p Antibody, NCUBE-1 Antibody, CGI-76 Antibody, UBC6 Antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family.

Western blot

Western blot
Anti-Ube2j1 Antibody - Western Blot. Western blot of affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over-expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.
 

Requested From: United States
Date Requested: 12/8/2016

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