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Anti-UBE2F Antibody (aa141-154) IHC-plus™ LS-B6482

Note: This antibody replaces LS-C108111
Catalog Size Price
LS-B6482-50 50 µg (0.5 mg/ml) Unavailable

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13 UBE2F Antibodies

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Anti-UBE2F Antibody (N-Terminus) LS-C81729
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Western blot Image
Anti-UBE2F Antibody (aa6-19) LS-C139772
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Anti-UBE2F Antibody (aa141-154) IHC-plus™ LS-B6482
Goat Polyclonal to Human UBE2F
Human, Monkey, Mouse
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-UBE2F Antibody LS-C489702
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100% Guaranteed
Goat Polyclonal to Human UBE2F
Human, Monkey, Mouse
IHC - Paraffin, Western blot, ELISA


Human UBE2F
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Bat, Bovine, Dog, Horse, Chicken, Zebrafish (at least 90% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Western blot (0.05 - 0.2 µg/ml)

Specificity and Use

UBE2F antibody was raised against synthetic peptide C-HLRDKEDFRNKVDD from the C-terminus of human UBE2F / UBE2F (NP_542409.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken, Lizard, Salmon, Zebrafish (93%); Mouse, Rat, Hamster, Elephant, Rabbit, Platypus (86%).
LS-E26886 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6482. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human UBE2F / UBE2F.
Western Blot: Approx. 20kD band observed in Mouse Liver lysates (calculated MW of 21.1kD according to Human NP_542409.1 and to Mouse NP_080730.1). Recommended concentration: 0.05-0.2 ug/ml.


Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About UBE2F

Q969M7 NP_542409.1

UBE2F Antibody, NCE2 Antibody, NEDD8 carrier protein UBE2F Antibody, NEDD8 protein ligase UBE2F Antibody, NEDD8-conjugating enzyme 2 Antibody, NEDD8-conjugating enzyme UBE2F Antibody

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.


Anti-UBE2F antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6482 concentration 2.5 ug/ml.

Western blot

Western blot
UBE2F antibody (0.05 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 1/22/2018

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