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Anti-UBE1L2 / UBE1L2 Antibody (aa300-350) IHC-plus™ LS-B1816

Note: This antibody replaces LS-C47400

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBE1L2 / UBE1L2 Antibodies

Anti-UBE1L2 / UBE1L2 Antibody (aa300-350) IHC-plus™ LS-B1816
Rabbit Polyclonal to Human UBE1L2 / UBE1L2
Human, Mouse, Rat, Bovine, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-UBE1L2 / UBE1L2 Antibody (clone 1D11) IHC-plus™ LS-B6351
Mouse Monoclonal [clone 1D11] (IgG2b,k) to Human UBE1L2 / UBE1L2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-UBE1L2 / UBE1L2 Antibody (aa775-825) LS-C148366
Rabbit Polyclonal to Human UBE1L2 / UBE1L2
Human
IHC - Paraffin, Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human UBE1L2 / UBE1L2
Human, Mouse, Rat, Bovine, Horse
IHC - Paraffin, Western blot
Unconjugated

Details

Human UBE1L2 / UBE1L2
Rabbit
Human, Mouse, Rat, Bovine, Horse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

UBE1L2 / UBE1L2 antibody was raised against a portion of amino acids 300-350 of human UBE1L2/MOP-4.
aa300-350
A portion of amino acids 300-350 of human UBE1L2/MOP-4.
Immunohistochemistry: LS-B1816 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1816 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About UBE1L2 / UBE1L2

A0AVT1 NM_018227 NP_060697.4

UBA6 Antibody, FLJ10808 Antibody, Monocyte protein 4 Antibody, MOP-4 Antibody, Ubiquitin-activating enzyme 6 Antibody, E1-L2 Antibody, MOP4 Antibody, UBE1L2 Antibody

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation.

Immunohistochemistry

Immunohistochemistry
Anti-UBA6 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1816 concentration 10 ug/ml.

Western blot

Western blot
Western blot of human UBE1L2/MOP-4 in Daudi cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW using antibody at 2 ug/ml.

Requested From: United States
Date Requested: 12/4/2016

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