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Anti-UBC / Ubiquitin C Antibody (aa36-66) IHC-plus™ LS-B3621

Note: This antibody replaces LS-C98777

Ordering

Wt. Vol. Conc. Price
- 200 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular UBC / Ubiquitin C Antibodies

Anti-UBC / Ubiquitin C Antibody LS-C2924
Rabbit Polyclonal
Human, Mouse, Bovine, Chicken, Drosophila, C. elegans
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-UBC / Ubiquitin C Antibody (aa1-32) IHC-plus™ LS-B3386
Rabbit Polyclonal to Human UBC / Ubiquitin C
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-UBC / Ubiquitin C Antibody (aa36-66) IHC-plus™ LS-B3621
Rabbit Polyclonal to Human UBC / Ubiquitin C
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human UBC / Ubiquitin C
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human UBC / Ubiquitin C
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

UBC / Ubiquitin C antibody was raised against kLH-conjugated synthetic peptide between amino acids 36-66 from C-terminal region of human Ubiquitin.
LS-E1560 - Lyophilized - 100 µg - $145.00
aa36-66
Immunohistochemistry: LS-B3621 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3621 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About UBC / Ubiquitin C

P0CG48 M26880 AAA36789.1

UBC Antibody, HUBCEP52 Antibody, L40 Antibody, HMG20 Antibody, RPL40 Antibody, Ubiquitin Antibody, Polyubiquitin-C Antibody, CEP52 Antibody, Ubiquitin C Antibody

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).

Immunohistochemistry

Immunohistochemistry
Anti-UBC / Ubiquitin C antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3621 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-UBC / Ubiquitin C antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3621 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Characterization of FOXP2 Isoforms. FOXP2.10t was detected with an antibody to the N-terminal XpressTM tag or FOXP2 (green) and counterstained with antibodies to the aggresome marker ubiquitin (red). Nuclei are marked by DAPI staining (blue). Ubiquitin co-localizes with FOXP2.10t aggregates suggesting that these cellular bodies represent aggresomes. (Hum. Mol. Genet. 2006 Nov 01;15(21):3154-3167)

Western blot

Western blot
Western blot of anti-Ubiquitin antibody in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Requested From: United States
Date Requested: 12/2/2016

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