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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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U2AF2 / U2AF65 Antibody (aa371‑420) LS‑C116802
U2AF65 antibody LS-C116802 is an unconjugated rabbit polyclonal antibody to U2AF65 (U2AF2) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C116802-50
50 µg
Unavailable

Popular U2AF2 / U2AF65 Products

Antibody
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Xenopus
Applications: Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: Western blot, ELISA
Immunofluorescence analysis of U2OS cells.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot
Immunofluorescent analysis of U2AF65 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

Product Description

U2AF65 antibody LS-C116802 is an unconjugated rabbit polyclonal antibody to U2AF65 (U2AF2) from human, mouse, rat and other species. Validated for WB.
About U2AF2 / U2AF65
Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. P26368 NM_007279 NP_009210.1

U2AF2 Antibody, HU2AF65 Antibody, HU2AF(65) Antibody, U2AF65 Antibody


Specifications

Target
Human U2AF2 / U2AF65
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
U2AF2 / U2AF65 antibody was raised against synthetic peptide located between aa371-420 of human U2AF2 (Q80XR5, NP_001012496). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Salmon, Stickleback, Zebrafish (100%); Xenopus (92%); Elephant, Sea squirt, Nematode (85%).
Blocking Peptide
LS-E14674 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116802. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa371-420
Specificity
Human U2AF2 / U2AF65
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
U2AF2 / U2AF65 antibody LS-C116802 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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