Human Tyrosinase-related Protein 1,2
(tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
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SK-MEL-23 melanoma cell line (human).
Recognizes human TRP-1,2 (tyrosinase-related protein-1,2). Detects a pigmentation-associated glycoprotein expressed by melanoma cells, normal melanocytes, and nevi. Does not detected in carcinomas or sarcomas. Species cross-reactivity: Human.
Suitable for use in Immunocytochemistry and Immunohistochemistry. Immunohistochemistry: 1:40 (Biotin). Fix frozen tissues and/or cytocentrifuge preparations in acetone at 4?C for 10 minutes). For optimal staining, the primary antibody should be incubated 20-60 minutes at RT. Positive control: Melanoma tissue. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.